I’ve been fortunate to have had many fantastic collaborators and learn many new things from them:
BioCAT (Srinivas Chakravarthy, Tom Irving and Jesse Hopkins): We have worked with BioCAT for nearly a decade. The development of technology pushing the time resolution of SAXS into the sub-100 microsecond regime is a direct product of this collaboration.
Venkatesh Inguva and Blair Perot (UMass-Amherst): Venkatesh and Blair were critical in developing the tools to simulate the flows in the microfluidic devices used in our continuous-flow experiments. They also devised and simulated new designs for more efficient mixing in the microsecond regime.
Dan Raleigh (Stony Brook University): We’ve been working with Dan’s group (primarily through his former grad student Ivan Peran) and Rohit Pappu’s group to get a better picture of the unfolded state using simulation (Alex Holehouse and Rohit) and small minimally perturbing fluorescent probes such as cyanophenylalanine. Our studies on Ntl9 and Ctl9 are nearing completion and should be out shortly.
Elisha Haas and Dan Amir (Bar Ilan University, Israel): We have had a long standing collaboration on microsecond continuous-flow experiments with TCSPC detection with Elisha’s group for a several years – it’s an honor to collaborate with the two people who carried out the first distance distribution analysis using time-resolved FRET on proteins. They were also the pioneers of double-kinetic experiments (experiments with two simultaneous time axes: one representing the excited state photophysics in nanoseconds and the other representing reaction kinetics of proteins in milliseconds to seconds).
Craig Peterson and Raushan Singh (UMass Med School): Raushan initiated our collaboration on using FCS-FRET to probe the influence of remodeling enzymes on nucleosome dynamics. We used the FCS-FRET approach originally proposed by the Levitus group to resolve dynamics in the microsecond to 10 ms time range. This work is out on bioRxiv.