Biophysics, protein science, spectroscopy, data analysis and instrumentation
smFRET/FCS microscope
In order to probe the conformation, dynamics, oligomeric state and kinetics of biological macromolecules we have built a custom single-molecule confocal microscope with time-correlated single-photon capabilities. The ‘scope has a number of features that make it convenient for probing protein-protein interactions:
488 nm picosecond diode laser excitation
594 nm CW laser excitation
idQuantique ultra-low-noise SPAD detectors with up to 4 channel capability (currently 2 are implemented)
Multi-SPC-150 TCSPC electronics
Fully automated
Custom xy-stage for automated measurements in microplates
Automatic coverslip finding algorithm
Automatic immersion water addition via Hamilton Microlab syringe pump
Power logging
Master control of all automation via custom LabVIEW software
